Gram staining technique was done to figure out the unknown gram positive shape (1) gram staining technique began with taking a sterile microscope slide and a sterile drop of distilled water and bacteria specimen by an inoculating loop. Start studying micro lab - report #15 - gram staining learn vocabulary, terms, and more with flashcards, games, and other study tools. A the gram stain discussion the gram stain is the most widely used staining procedure in bacteriology it is called a differential stain since it differentiates between gram-positive and gram-negative bacteria. Microscopic examination remains the initial diagnostic test in the processing of specimens in the clinical microbiology laboratory the timely report of a gram stain result gives the physician important information about the presence and cause of infection.
Gram staining is a bacteriological laboratory technique used to differentiate bacterial species into two large groups (gram-positive and gram-negative) based on the physical properties of their cell walls. The significance of the gram staining procedure is that it differentiates the bacteria into two groups the gram reaction of a bacterium adds valuable information for the treatment of disease the endospore stain is a special stain that is used to determine the presence of endospores in bacteria. The gram stain remains a major tool in the clinical microbiology lab al, chapter 3 and about gram positive and negative cells and their cell walls in chapter 4, especially • differential stain medical laboratory observer [serial on the internet.
Study flashcards on lab exercise 7: the gram stain at cramcom quickly memorize the terms, phrases and much more cramcom makes it easy to get the grade you want. A gram stain was taken from a sample of the bacteria growing on the nutrient agar the gram stain turned out to be a positive rod gram stain all the following procedures were carried out on the gram positive rod unknown in this order. The gram stain procedure was performed as directed in the lab manual (1) the gram stain showed a result of red, gram negative rods to decipher between which biochemical tests to perform, the gram positive and negative tables handed out by the professor, were referred to. After the morphology was observed and recorded, a gram stain was performed on each, this procedure is found in the laboratory manual the two microorganisms were identified one was a gram-positive bacteria, the other was a gram-negative bacteria. For more information, visit this video demonstrates the gram staining method for identifying unknown bacter.
Fig 1 gram stain interpretation proficiencies of three satellite laboratories, determined by quality assurance program 1 and delineated by interpretation of bacteria (black bars) and host cells (white bars) and the sum of the two categories (shaded bars. The lab instructor provided information that one species was a gram-positive bacteria and the other species was a gram-negative therefore, the next necessary step for correct identification of each unknown was to complete a gram staining procedure described on page 67 of the lab manual (2. The gram stain is a differential staining technique used to classify & categorize bacteria into two major groups: gram positive and gram negative, based on the differences of the chemical and physical properties of the cell wall. That most bacteria are transparent and cannot be seen through the microscope the gram stain is a type of differential stain that allows a (micro)biologist to water was placed on a microscope slide and bacteria from unknown number 20 were also placed on the slide then the slide had to be air dried. Unknown lab report unknown number 117 joel hentrich april 29 th, 2014 microbiology spring 2014 introduction the purpose of this study was multifaceted: first, it was completed in order to gain a better understanding of how to utilize microbiological techniques learned within the classroom and laboratory environment.
• to learn the techniques of gram staining, nigrosin staining and koh test • to use and relate the gram stain to the study of bacterial cell morphology, and as an important step in the identification of a bacterial species. The gram stain is one of the most important methods used in the clinical microbiology laboratory it is used for initial assessment of specimens prior to culture and is an important step in the workup and identification of pathogens isolated from clinical specimens. Introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory for microscopy essay the format of the lab report is tailored to each institution or outpatient contract laboratory, although the. Gram stain lab report by professional college essay ghostwriter for hire for university | help me write a story | 30 march, 2016 custom university essay writer service for mba, esl dissertation hypothesis writers sites ca. (ocular lens, 10x): -the lab microscope is a binocular microscope-objects can view with both eyes --adjust the distance between the oculars by opening or closing the gap between them until you can see equally through both.
Gram staining is a very important technique used in biology labs all over the world it is a technique used to differentiate types of bacteria using certain physical and chemical characteristics of their cell walls. This is the main page for the gram stain & isolation streak plate laboratory topic of an 8-week introductory college microbiology class the links on this page provide the virtual microbiology classroom documents, web pages and videos useful in preparing for this lab. Unknown lab report unknown number 109 tyler wolfangel april 29, 2014 bio 203-604 introduction the study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. We performed a two-part experiment to gain a better understanding of bacteria through gram-staining gram staining is a differential staining method which results in the classification of two groups of bacteria, gram-positive and gram-negative.
The gram stain is a pivotal stain in microbiology this stain should be repeated when pure cultures are isolated lab 2b: introduction to streaking and culture maintenance. Amanda lab report 1 microbiology laboratory title - differentiating organisms using the gram stain introduction the experiment conducted was based upon the known attributes of two different groups of bacteria, those that are gram positive, and those that are gram negative.
The gram stain is a differential stain that utilizes a primary stain of crystal violet (ie a purple dye), iodine, alcohol decolorization, and safranin (ie a tinted red dye) used as counterstain standard results of a proper gram stain would show a bacterium to be gram positive if purple, and gram negative if pink. In this lab you will be innoculating plates and observing bacterial growth microscopes can then be used to identify specific bacteria this lab may take several days, keep all data and observations in a separate notebook to be compiled and organized into a final lab report.